Biochemical Approaches for Glutamatergic Neurotransmission
This volume presents techniques and recent developments in biochemical approaches to study glutamatergic neurotransmission. This book contains detailed discussions on tracing neuronal pathways, functional or spectroscopic imaging, optogenetic or pharmacological tools, and extracellular neurochemistry in experimental clinical models. The chapters cover topics such as: identification of glutamatergic neurons in a discrete brain area, tools to study dynamics of single ionotropic receptors, in vivo extracellular glutamate monitoring in preclinical research using biosensors, capillary electrophoresis and liquid chromatography, and potential brain glutamatergic clinical biomarkers in biofluids and tissues. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and authoritative, Biochemical Approaches for Glutamatergic Neurotransmission is a valuable resource for researchers studying glutamate neurochemistry.
InhoudSeries Preface... Preface... Table of Contents... Contributing Authors...1. Identification of Glutamatergic NeuronsShiliang Zhang and Marisela Morales2. Single Nanoparticle Tracking: A Method for Investigating the Surface Dynamics of Glutamate ReceptorsJulien Dupuis, Francois Maingret, and Laurent Groc3. Positron Emission Tomography of Metabotropic Glutamate ReceptorsSelena Milicevic Sephton4. NMR Spectroscopy of Brain Glutamate FunctionJun Shen5. Imaging Glutamate with Genetically Encoded Fluorescent SensorsGerard J. Broussard, Elizabeth K. Unger, Ruqiang Liang, Brian P. McGrew, and Lin Tian6. Measurement of Astrocytic Glutamate Release using Genetically-Encoded Probe Combined with TIRF MicroscopyFuyuko Takata-Tsuji, Naura Choulnamountri, Christophe Place, and Olivier Pascual7. Drugs to Alter Extracellular Concentration of Glutamate: Modulators of Glutamate Uptake SystemsAndreia Cristina Karklin Fontana8. Drugs to Tune Up Glutamatergic Systems: Modulators of Glutamate Metabotropic ReceptorsKathy Sengmany and Karen J. Gregory9. Glutamatergic Synthesis, Recycling, and Receptor Pharmacology at Drosophila and Crustacean Neuromuscular JunctionsJoshua S. Titlow and Robin L. Cooper10. Optical Control of Glutamate Receptors of the NMDA-Kind in Mammalian Neurons, with the use of Photoswitchable LigandsShai Berlin and Ehud Y. Isacoff11. In Vivo Electrochemical Studies of Optogenetic-Control of Glutamate Signaling Measured Using Enzyme-Based Ceramic Microelectrode ArraysJason J. Burmeister, Francois Pomerleau, Jorge E. Quintero, Peter Huettl, Yi Ai, Johan Jakobsson, Martin Lundblad, Andreas Heuer, John T. Slevin, and Greg A. Gerhardt12. Separation-Based Sensors using Microchip Electrophoresis with Microdialysis for Monitoring Glutamate and Other Bioactive AminesNathan Oborny, Michael Hogard, and Susan Lunte13. Brain Glutamate Monitoring by Microdialysis and Separation Methods with a Special Focus on Capillary Electrophoresis with Laser-Induced Fluorescence DetectionLuc Denoroy and Sandrine Parrot14. In Vivo Determination of Glutamate Uptake by Brain MicrodialysisSandrine Parrot, Monique Touret, and Luc Denoroy15. Enzymes of Glutamate SystemIrina S. Boksha, Olga K. Savushkina, Elena B. Tereshkina, Tatyana A. Prokhorova, and Elizabeta B. Mukaetova-Ladinska16. The Glutamatergic System as Potential Clinical Biomarkers for Blood and Cerebrospinal Fluid MonitoringKenji Hashimoto17. Clinical CNS Microdialysis of Glutamate with a Special Methodological Focus on Human Spinal CordBernard Renaud, Luc Denoroy, Delphine Collin-Chavagnac, Patrick Mertens, and Sandrine Parrot