This volume describes up-to-date techniques used in understanding the molecular biology of acute myeloid leukemia (AML) and addressing advances in diagnosis, classification, prognostication, and therapeutic strategies to potentially impact overall patient survival. The chapters in this book cover topics such as: cytochemical staining, single-cell mass cytometry of AML and Leukemia stem/progenitor cells, microsphere-based assessment of DNA methylation for AML prognosis, a zebrafish model for evaluating the function of human leukemic gene IDH1 and its mutation, and the isolation of biologically active exosomes from plasma of patients with cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Acute Myeloid Leukemia: Methods and Protocols is a valuable resource for scientists and researchers to further their studies and advancements in the field of AML.
Preface... Table of Contents... Contributing Authors... 1. Molecular Malfeasance Mediating Myeloid Malignancies: The Genetics of Acute Myeloid Leukemia Rebecca L. King and Adam Bagg 2. Cytochemical Staining Michele E. Paessler, Marybeth Helfrich, and Gerald B. Wertheim 3. Immunohistochemistry in Acute Myeloid Leukemia Michael W. Cruise 4. Immunophenotyping by Multiparameter Flow Cytometry Weina Chen and Hung S. Luu 5. Single-Cell Mass Cytometry of Acute Myeloid Leukemia and Leukemia Stem/Progenitor Cells Zhihong Zeng, Marina Konopleva, and Michael Andreeff 6. Design and Application of Multiplex-PCR-Seq for the Detection of Somatic Mutations Associated with Myeloid Malignancies Naomi Park and George Vassiliou 7. AMLProfiler: A Diagnostic and Prognostic Microarray for Acute Myeloid Leukemia Marco Alessandrini, Sharon S. Kappala, and Michael S. Pepper 8. Microsphere-Based Assessment of DNA Methylation for AML Prognosis Gerald B. Wertheim, marlise R. Luskin, Martin Carroll, and Stephen R. Master 9. Genome-Wide Analysis of DNA Methylation in Hematopoietic Cells: DNA Methylation Analysis by WGBS Mira Jeong, Anna G. Guzman, and Margaret A. Goodell 10. Detection and Quantification of Acute Myeloid Leukemia-Associated Fusion Transcripts Jonathan Schumacher, Philippe Szankasi, and Todd W. Kelley 11. Targeted Next-Generation Sequencing of Acute Leukemia Eric Konnick, Christina M. Lockwood, and David Wu 12. Bioinformatics Data Analysis of Next-Generation Sequencing Data from Heterogeneous Tumor Samples Sean R. Landman and Tae Hyun Hwang 13. A Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and its Mutation Alvin C.H. Ma, Xiangguo Shi, Bai-Liang He, Yuhan Guo, and Anskar Y.H. Leung 14. Measurement of Oncometabolites D-2-Hydroxyglutaric Acid and L-2-Hydroxyglutaric Acid Patricia M. Jones, Richard Boriack, Eduard A. Struys, and Dinesh Rakheja 15. Nanofluidic Allele-Specific Digital PCR Method for Quantifying IDH1 and IDH2 Mutation Burden in Acute Myeloid Leukaemia Daniel H. Wiseman and Tim C.P. Somervaille 16. Isolation of Biologically Active Exosomes from Plasma of Patients with Cancer Chang-Sook Hong, Sonja Funk, and Theresa L. Whiteside 17. Generating and Expanding Autologous Chimeric Antigen Receptor T Cells from Patients with Acute Myeloid Leukemia Saad S. Kenderian, Carl H. June, and Saar Gill